Common Problems Encountered During Production of Recombinant Proteins and their Solutions

Production of Recombinant Proteins

Introduction Recombinant Protein Recombinant protein is a modified form of protein, which is generated in many ways to produce large quantities of proteins, alter gene sequences and produce useful commercial products. The formation of recombinant protein is carried out in specialized vehicles known as vectors. Recombinant protein is a protein encoded by a gene — recombinant DNA — that has been cloned in a system that supports the expression of the gene and the translation of messenger RNA. Proteins co-expressed in bacteria will not have post-translational modifications, e.g. phosphorylation or…

Bioprocessing of VLPs used in vaccine production

Bioprocessing of VLPs used to produce veccines

History of VLPs: (As compounds of advanced vaccine Strategies) Bioprocessing of VLPs | Gofrixty | Previous to 1969 anti-viral vaccines were either derived from inactivated viruses such as rabies vaccine, attenuated strains of pathogenic wild type viruses such as (yellow fever or poliovirus) or they were using non-pathogenic viral strains to persuade a defending immune response against pathogen family members like a vaccine of smallpox containing harmless vaccinia virus. Since then particles based on HBsAg were firstly revealed in the blood samples of patients infected with HBV and soon were…

Asad Umar’s mathematics of the recent petrol price hike

Asad Umar mathematics of the recent petrol price hike comment of Murtaza wahab

Since yesterday a hashtag of RS 5 was trending on Twitter in Pakistan. That was because of the recent increase in the price of the patrol by Rs 5 by Pakistan’s governing bodies. In response to the recent price hike, Murtaza Wahab Siddiqui, Advisor to Chief Minister Sindh on Law & Environment, Spokesperson Sindh Government – Deputy Information Secretary, PPP Sindh and Former Senator, commented on Asad Umar’s Tweet that he published on February 1, 2016. Asad Umar wrote when he and his party (PTI) was in opposition and PML(N)…

Mullah Baradar & Narendra Modi among Time’s 100 most influential people 2021

Mullah Baradar Narendra Modi and Imran Khan 100 most influential list of 2021

Mullah Baradar Abdul Ghani’s name has been listed in the Time’s 100 most influential people 2021 list. Along with Mullah Baradar, there is a list of some notable people who are also being listed as world’s 100 most influencing personalities. India’s Prime Minister Narendra Modi and Afghanistan’s Women’s rights activist Mahbouba Seraj are also among the list of 100 top influencers of 2021. Afghanistan’s charismatic military leader and co-founder of Taliban, Mullah Baradar, is a deeply pious personality. Currently, the people of Afghanistan are now at the verge of Mullah…

USDT into PKR | September 14, 2021 | Cryptocurrency rates

USDT into PKR 14 September 2021 2021

Latest cryptocurrency rates update | USDT into PKR | Gofrixty Daily Crypto | September 14, 2021 | 09:00 +05:00 GMT | Tether, ISO code USDT, is a stable coin or often named stable currency that converts cash into digital currency. As per the coin market cap, it is the fourth largest crypto asset after Bitcoin, Ethereum, and Cardano Coins. The latest update of the conversion rate of the USDT into Pakistani currency as of September 14, 2021, i.e. Tuesday is Rs. 167.96 as of coin market cap. Advertisement According to…

The educational system of Sindh | A systematic review

The education system of Sindh

“Sindh has fallen behind the world in terms of quality education, as the demand for better schools is growing rapidly while the supply of quality schools is just not keeping pace.” Education plays an important role in the development and prosperity of the country. The quality of education is the backbone of national progress. As Christine Gregoire said: “Education is the foundation of our future.” If we look back at history, we have an example. Japan, a country that completely declined after the war, but as the saying goes, “bless…

Shah Rukh Khan’s Daughter Looks Stunning In Her New Look

Shah Rukh Khan’s Daughter Suhana Khan looks stunning in her new pics on Instagram. People follow her because of her attractive looks. Shah Rukh Khan is a global star, one of the most famous personalities of the world. His daughter is currently studying Showbiz in America. He said many times that Suhana wants to join the industry in future. She frequently shares her photos on the Instagram. Sometimes she share pictures with her friends. She is into the spotlight for quite long. She already has 1.7 million followers on instagram,…

All Pakistani Citizens Can Now Register For Covid-19 Vaccination

Covid Vaccination

National command and operation centre NCOC has finally announced the registration of vaccine for all the Pakistani citizens of 30 and above to get vaccinated by tomorrow, Sunday 16th May. Minister of planning and development Asad Umer finally took to the twitter and stated: “As vaccine supply continues to improve and vaccination capacity gets enhanced in all Federating units, we continue to expand the categories of those who are eligible to get vaccinated.” Advertisement Keeping in mind that, vaccination centres are open now after closing for the first two days…

WhatsApp new app policies | users will lose some app feature if they don’t accept its new policies

WhatsApp new app policies 2021

Gofrixty News Desk | 11 May 2021 | WhatsApp new app policies | WhatsApp as of late presented new protection strategies for its users, which incorporates new terms guaranteeing that the application will impart information to Facebook as a state of utilization. Albeit this was delayed from the outset, WhatsApp will presently handicap some application functionalities until clients acknowledge the new security terms. Following the discussions that WhatsApp new app policies arrangements have caused, Facebook has chosen to defer changes with respect to information dividing among the two interpersonal organizations.…

Amazon approved Pakistan in its seller list 2021

Pakistan Amazon Seller Amazon approved Pakistan in selling list 2021

Pakistan has finally been approved to be on the seller list of amazon. Amazon has officially announced to establish a platform in Pakistan and some other countries too. People who own a business or tend to start a business in Pakistan will get to avail the opportunity within a couple of days through Pakistan amazon seller. What is Amazon? Amazon is an international e-commerce platform to buy or sell products worldwide. It has a high-quality customer base. Because around the world, people sell their goods on this platform to any…